| 20. Round | 05/04/2025 | Viktoria Plzeň [Youth] | - | FK Dukla Praha [Youth] | 0:2 | ||||
| 21. Round | 12/04/2025 | FK Dukla Praha [Youth] | - | Vysočina Jihlava [Youth] | 2:1 | ||||
| 23. Round | 26/04/2025 | FK Dukla Praha [Youth] | - | FK Pardubice [Youth] | 2:3 | ||||
| 25. Round | 10/05/2025 | FK Dukla Praha [Youth] | - | FK Jablonec [Youth] | 3:2 | ||||
| 24. Round | 13/05/2025 | Slavia Praha [Youth] | - | FK Dukla Praha [Youth] | 3:5 | ||||
| 26. Round | 17/05/2025 | Slovan Liberec [Youth] | - | FK Dukla Praha [Youth] | 0:0 | ||||
| 27. Round | 24/05/2025 | FK Dukla Praha [Youth] | - | SFC Opava [Youth] | 3:1 | ||||
| 28. Round | 31/05/2025 | 1. FC Slovácko [Youth] | - | FK Dukla Praha [Youth] | 2:5 |  |
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| 30. Round | 11/06/2025 | FK Dukla Praha [Youth] | - | Sigma Olomouc [Youth] | 0:0 |
Czech Republic