| 1. Round | 06/08/2022 | Vysočina Jihlava [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 | ||||
| 2. Round | 13/08/2022 | 1. FC Slovácko [Youth B] | - | FK Pardubice [Youth B] | 3:1 |  |
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| 3. Round | 19/08/2022 | Banik Ostrava [Youth B] | - | 1. FC Slovácko [Youth B] | 4:3 | ||||
| 4. Round | 27/08/2022 | 1. FC Slovácko [Youth B] | - | Dynamo České Budějovice [Youth B] | 4:0 | ||||
| 12. Round | 21/10/2022 | MFK Karviná [Youth B] | - | 1. FC Slovácko [Youth B] | 0:3 | |
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| 14. Round | 26/10/2022 | FC Zlín [Youth B] | - | 1. FC Slovácko [Youth B] | 1:1 | ||||
| 13. Round | 29/10/2022 | 1. FC Slovácko [Youth B] | - | FK Příbram [Youth B] | 9:2 | |
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| 24. Round | 29/04/2023 | FC Zbrojovka Brno [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 |
Czech Republic