| 10. Round | 03/10/2009 | 1. FC Slovácko | - | Baník Ostrava | 0:2 | ||||
| 11. Round | 18/10/2009 | Bohemians 1905 | - | 1. FC Slovácko | 0:1 | ||||
| 12. Round | 24/10/2009 | 1. FC Slovácko | - | SK Kladno | 2:0 |  |
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| 13. Round | 01/11/2009 | FK Mladá Boleslav | - | 1. FC Slovácko | 3:2 | ||||
| 14. Round | 09/11/2009 | 1. FC Slovácko | - | AC Sparta Praha | 0:1 | ||||
| 15. Round | 21/11/2009 | 1. FK Příbram | - | 1. FC Slovácko | 2:0 | ||||
| 16. Round | 28/11/2009 | 1. FC Slovácko | - | FK Baumit Jablonec | 1:1 | ||||
| 18. Round | 06/03/2010 | 1. FC Slovácko | - | 1. FC Brno | 0:2 | ||||
| 19. Round | 14/03/2010 | Bohemians Praha | - | 1. FC Slovácko | 1:1 |  |
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| 22. Round | 28/03/2010 | 1. FC Slovácko | - | Dynamo České Budějovice | 1:0 | ||||
| 23. Round | 03/04/2010 | 1. FC Slovácko | - | Slovan Liberec | 0:0 | ||||
| 25. Round | 17/04/2010 | 1. FC Slovácko | - | Bohemians 1905 | 1:1 | ||||
| 26. Round | 25/04/2010 | SK Kladno | - | 1. FC Slovácko | 1:0 | |
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| 27. Round | 30/04/2010 | 1. FC Slovácko | - | FK Mladá Boleslav | 1:0 | ||||
| 28. Round | 05/05/2010 | AC Sparta Praha | - | 1. FC Slovácko | 4:0 | ||||
| 29. Round | 08/05/2010 | 1. FC Slovácko | - | 1. FK Příbram | 2:1 | |
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| 30. Round | 15/05/2010 | Viktoria Plzeň | - | 1. FC Slovácko | 2:2 |
Czech Republic