| 16. Round | 02/03/2024 | FK Mladá Boleslav [Youth B] | - | 1. FC Slovácko [Youth B] | 3:3 | ||||
| 17. Round | 09/03/2024 | 1. FC Slovácko [Youth B] | - | Slovan Liberec [Youth B] | 0:3 | ||||
| 20. Round | 30/03/2024 | FK Frýdek-Místek [Youth B] | - | 1. FC Slovácko [Youth B] | 1:1 |  |
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| 19. Round | 03/04/2024 | 1. FC Slovácko [Youth B] | - | Slavia Praha [Youth B] | 1:5 | |
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| 21. Round | 06/04/2024 | 1. FC Slovácko [Youth B] | - | Banik Ostrava [Youth B] | 1:9 | ||||
| 22. Round | 12/04/2024 | MFK Karviná [Youth B] | - | 1. FC Slovácko [Youth B] | 4:2 |  |
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| 23. Round | 20/04/2024 | 1. FC Slovácko [Youth B] | - | Viktoria Plzeň [Youth B] | 1:4 | ||||
| 24. Round | 27/04/2024 | AC Sparta Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 2:2 | ||||
| 25. Round | 04/05/2024 | Sigma Olomouc [Youth B] | - | 1. FC Slovácko [Youth B] | 4:0 | ||||
| 26. Round | 11/05/2024 | 1. FC Slovácko [Youth B] | - | FK Pardubice [Youth B] | 0:6 | ||||
| 27. Round | 18/05/2024 | FC Zlín [Youth B] | - | 1. FC Slovácko [Youth B] | 1:2 | |
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| 29. Round | 01/06/2024 | Vysočina Jihlava [Youth B] | - | 1. FC Slovácko [Youth B] | 2:2 | ||||
| 30. Round | 08/06/2024 | 1. FC Slovácko [Youth B] | - | FK Dukla Praha [Youth B] | 0:3 |
Czech Republic