| 1. Round | 11/08/2024 | FK Admira Praha | - | FK Dukla Praha B | 1:0 |  |
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| 2. Round | 18/08/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 2:0 | ||||
| 3. Round | 24/08/2024 | TJ Jiskra Domažlice | - | FK Dukla Praha B | 3:1 | ||||
| 17. Round | 28/08/2024 | FK Dukla Praha B | - | Viktoria Plzeň B | 2:0 | |
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| 4. Round | 01/09/2024 | FK Dukla Praha B | - | Povltavská FA | 1:4 | ||||
| 12. Round | 27/10/2024 | FK Dukla Praha B | - | FK MAS Táborsko B | 2:3 | ||||
| 13. Round | 01/11/2024 | FC Písek | - | FK Dukla Praha B | 1:1 | ||||
| 16. Round | 23/11/2024 | Sokol Hostouň | - | FK Dukla Praha B | 2:5 | ||||
| 21. Round | 31/03/2025 | Bohemians Praha 1905 B | - | FK Dukla Praha B | 2:0 | |
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| 32. Round | 07/05/2025 | FK Dukla Praha B | - | Dynamo České Budějovice B | 0:2 |
Albania