| 3. Round | 17/08/2024 | 1. FC Slovácko B | - | FK Frýdek-Místek | 1:0 | ||||
| 10. Round | 05/10/2024 | FK Blansko | - | 1. FC Slovácko B | 2:0 | ||||
| 13. Round | 27/10/2024 | 1. FC Slovácko B | - | SK Uničov | 4:1 |  |
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| 7. Round | 06/11/2024 | 1. FC Slovácko B | - | MFK Karviná B | 1:2 | ||||
| 18. Round | 09/03/2025 | 1. FC Slovácko B | - | FK Hodonín | 2:2 | ||||
| 21. Round | 30/03/2025 | 1. FC Slovácko B | - | FC Slovan Rosice | 2:0 | |
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| 22. Round | 05/04/2025 | TJ Start Brno | - | 1. FC Slovácko B | 0:1 | ||||
| 23. Round | 13/04/2025 | 1. FC Slovácko B | - | FC Hlučín | 1:0 | ||||
| 24. Round | 18/04/2025 | MFK Karviná B | - | 1. FC Slovácko B | 0:2 | |
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| 25. Round | 27/04/2025 | 1. FC Slovácko B | - | FC Zlín B | 1:0 | |
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| 33. Round | 30/04/2025 | 1. FC Slovácko B | - | TJ Unie Hlubina | 0:1 | ||||
| 26. Round | 04/05/2025 | FC Zbrojovka Brno B | - | 1. FC Slovácko B | 1:2 | ||||
| 27. Round | 11/05/2025 | 1. FC Slovácko B | - | FK Blansko | 0:4 | ||||
| 28. Round | 17/05/2025 | ČSK Uherský Brod | - | 1. FC Slovácko B | 2:1 |
Slovakia