| 1. Round | 04/08/2023 | MFK Karviná [Youth B] | - | FC Zbrojovka Brno [Youth B] | 1:6 | ||||
| 3. Round | 19/08/2023 | MFK Karviná [Youth B] | - | FK Frýdek-Místek [Youth B] | 4:1 |  |
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| 6. Round | 09/09/2023 | MFK Karviná [Youth B] | - | Viktoria Plzeň [Youth B] | 1:2 | ||||
| 7. Round | 16/09/2023 | 1. FC Slovácko [Youth B] | - | MFK Karviná [Youth B] | 2:2 | ||||
| 8. Round | 22/09/2023 | MFK Karviná [Youth B] | - | Sigma Olomouc [Youth B] | 0:4 | ||||
| 12. Round | 21/10/2023 | MFK Karviná [Youth B] | - | Vysočina Jihlava [Youth B] | 1:1 |  |
Czech Republic