| 1. Round | 05/08/2023 | FK Loko Vltavín | - | FK MAS Táborsko B | 1:1 |  |
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| 2. Round | 12/08/2023 | TJ Jiskra Domažlice | - | FK Loko Vltavín | 2:1 | ||||
| 3. Round | 19/08/2023 | FK Loko Vltavín | - | Slavia Praha B | 1:3 | ||||
| 4. Round | 27/08/2023 | Sokol Hostouň | - | FK Loko Vltavín | 2:1 | ||||
| 5. Round | 02/09/2023 | FK Loko Vltavín | - | FK Admira Praha | 4:1 | |
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| 6. Round | 09/09/2023 | FK Králův Dvůr | - | FK Loko Vltavín | 0:3 | ||||
| 7. Round | 16/09/2023 | FK Loko Vltavín | - | Dynamo České Budějovice B | 2:5 | ||||
| 8. Round | 23/09/2023 | FK Loko Vltavín | - | Povltavská FA | 1:1 | ||||
| 9. Round | 30/09/2023 | FK Robstav | - | FK Loko Vltavín | 5:0 | ||||
| 10. Round | 07/10/2023 | FK Loko Vltavín | - | Viktoria Plzeň B | 1:1 | ||||
| 11. Round | 14/10/2023 | Slavia Karlovy Vary | - | FK Loko Vltavín | 2:1 | ||||
| 12. Round | 21/10/2023 | FK Loko Vltavín | - | FK Dukla Praha B | 0:0 | ||||
| 13. Round | 28/10/2023 | FK Motorlet Praha | - | FK Loko Vltavín | 2:2 | ||||
| 14. Round | 04/11/2023 | FK Loko Vltavín | - | Bohemians Praha 1905 B | 2:2 | ||||
| 15. Round | 10/11/2023 | FC Písek | - | FK Loko Vltavín | 3:2 | |
Czech Republic