| 1. Round | 09/08/2024 | FC Zlín [Youth B] | - | Vysočina Jihlava [Youth B] | 2:2 | ||||
| 2. Round | 16/08/2024 | FK Mladá Boleslav [Youth B] | - | FC Zlín [Youth B] | 4:1 | ||||
| 4. Round | 30/08/2024 | Slavia Praha [Youth B] | - | FC Zlín [Youth B] | 9:1 | ||||
| 5. Round | 07/09/2024 | FC Zlín [Youth B] | - | FK Jablonec [Youth B] | 3:0 | ||||
| 8. Round | 28/09/2024 | 1. FC Slovácko [Youth B] | - | FC Zlín [Youth B] | 0:1 | ||||
| 12. Round | 29/10/2024 | FC Zlín [Youth B] | - | Banik Ostrava [Youth B] | 1:4 | ||||
| 13. Round | 02/11/2024 | Dynamo České Budějovice [Youth B] | - | FC Zlín [Youth B] | 1:1 | ||||
| 14. Round | 09/11/2024 | FC Zlín [Youth B] | - | FK Dukla Praha [Youth B] | 1:2 |  |
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| 15. Round | 16/11/2024 | Viktoria Plzeň [Youth B] | - | FC Zlín [Youth B] | 2:2 | ||||
| 7. Round | 23/11/2024 | FC Zlín [Youth B] | - | SFC Opava [Youth B] | 6:3 |
Czech Republic