| 2. Round | 02/08/2009 | SK Kladno | - | 1. FK Příbram | 0:2 | ||||
| 4. Round | 15/08/2009 | AC Sparta Praha | - | SK Kladno | 2:0 | ||||
| 7. Round | 13/09/2009 | SK Kladno | - | 1. FC Brno | 1:0 | ||||
| 8. Round | 20/09/2009 | Sigma Olomouc | - | SK Kladno | 1:2 | ||||
| 10. Round | 04/10/2009 | Dynamo České Budějovice | - | SK Kladno | 0:0 | ||||
| 13. Round | 01/11/2009 | SK Kladno | - | FK Teplice | 0:2 | ||||
| 15. Round | 21/11/2009 | SK Kladno | - | Bohemians 1905 | 1:2 |  |
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| 16. Round | 28/11/2009 | 1. FK Příbram | - | SK Kladno | 3:2 | ||||
| 17. Round | 28/02/2010 | FK Mladá Boleslav | - | SK Kladno | 0:1 |  |
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| 18. Round | 07/03/2010 | SK Kladno | - | AC Sparta Praha | 0:1 | ||||
| 20. Round | 20/03/2010 | SK Kladno | - | Viktoria Plzeň | 1:3 | ||||
| 21. Round | 24/03/2010 | 1. FC Brno | - | SK Kladno | 3:0 | ||||
| 22. Round | 28/03/2010 | SK Kladno | - | Sigma Olomouc | 2:3 | ||||
| 23. Round | 04/04/2010 | Bohemians Praha | - | SK Kladno | 1:4 | ||||
| 24. Round | 11/04/2010 | SK Kladno | - | Dynamo České Budějovice | 2:1 | ||||
| 27. Round | 01/05/2010 | FK Teplice | - | SK Kladno | 5:0 | ||||
| 30. Round | 15/05/2010 | SK Kladno | - | Slovan Liberec | 1:2 |
Czech Republic