| 18. Round | 09/03/2025 | MFK Karviná B | - | SK Uničov | 0:1 | ||||
| 21. Round | 29/03/2025 | TJ Unie Hlubina | - | MFK Karviná B | 2:4 | ||||
| 23. Round | 12/04/2025 | FK Hodonín | - | MFK Karviná B | 1:0 | ||||
| 33. Round | 30/04/2025 | MFK Karviná B | - | ČSK Uherský Brod | 4:2 | ||||
| 30. Round | 31/05/2025 | FC Zlín B | - | MFK Karviná B | 3:2 |  |
Poland