| 1. Round | 04/08/2024 | SK Uničov | - | MFK Karviná B | 2:1 | ||||
| 2. Round | 11/08/2024 | FC Zlín B | - | SK Uničov | 3:1 | ||||
| 3. Round | 18/08/2024 | SK Uničov | - | FC Zbrojovka Brno B | 7:3 | ||||
| 4. Round | 23/08/2024 | FK Blansko | - | SK Uničov | 1:1 | ||||
| 5. Round | 01/09/2024 | SK Uničov | - | ČSK Uherský Brod | 2:0 | ||||
| 6. Round | 06/09/2024 | FC Zlínsko | - | SK Uničov | 1:3 | ||||
| 17. Round | 11/09/2024 | FC Hlučín | - | SK Uničov | 0:1 | ||||
| 14. Round | 03/11/2024 | SK Uničov | - | FK Frýdek-Místek | 2:1 | ||||
| 15. Round | 09/11/2024 | FC Slovan Rosice | - | SK Uničov | 0:3 |
Czech Republic