| 1. Round | 17/08/2024 | FK Dukla Praha [Women] | - | SK Líšeň [Women] | 2:2 | ||||
| 2. Round | 24/08/2024 | Prague Raptors FC [Women] | - | FK Dukla Praha [Women] | 1:2 | ||||
| 3. Round | 31/08/2024 | FK Dukla Praha [Women] | - | FK Teplice [Women] | 1:2 | ||||
| 4. Round | 08/09/2024 | Dynamo České Budějovice [Women] | - | FK Dukla Praha [Women] | 3:0 | ||||
| 6. Round | 21/09/2024 | Sigma Olomouc [Women] | - | FK Dukla Praha [Women] | 6:0 | ||||
| 7. Round | 28/09/2024 | FK Dukla Praha [Women] | - | AC Sparta Praha B [Women] | 1:4 | ||||
| 8. Round | 06/10/2024 | FC Zlín [Women] | - | FK Dukla Praha [Women] | 0:2 | ||||
| 9. Round | 12/10/2024 | FK Dukla Praha [Women] | - | FC Hradec Králové [Women] | 1:2 | ||||
| 13. Round | 09/03/2025 | FK Dukla Praha [Women] | - | Dynamo České Budějovice [Women] | 2:0 | ||||
| 14. Round | 15/03/2025 | Baník Ostrava [Women] | - | FK Dukla Praha [Women] | 5:1 | ||||
| 15. Round | 23/03/2025 | FK Dukla Praha [Women] | - | Sigma Olomouc [Women] | 2:3 |  |
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| 16. Round | 30/03/2025 | AC Sparta Praha B [Women] | - | FK Dukla Praha [Women] | 7:0 |
Czech Republic