| 1. Round | 11/08/2024 | FK Admira Praha | - | FK Dukla Praha B | 1:0 |  |
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| 2. Round | 18/08/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 2:0 |  |
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| 3. Round | 24/08/2024 | TJ Jiskra Domažlice | - | FK Dukla Praha B | 3:1 | ||||
| 17. Round | 28/08/2024 | FK Dukla Praha B | - | Viktoria Plzeň B | 2:0 | |
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| 4. Round | 01/09/2024 | FK Dukla Praha B | - | Povltavská FA | 1:4 | |
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| 7. Round | 20/09/2024 | FK Příbram | - | FK Dukla Praha B | 2:2 | |
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| 8. Round | 29/09/2024 | FK Dukla Praha B | - | Slovan Velvary | 1:1 | ||||
| 9. Round | 05/10/2024 | FK Motorlet Praha | - | FK Dukla Praha B | 2:0 | ||||
| 10. Round | 13/10/2024 | FK Dukla Praha B | - | Bohemians Praha 1905 B | 4:0 | ||||
| 11. Round | 19/10/2024 | SK Petřín Plzeň | - | FK Dukla Praha B | 0:2 | |
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| 12. Round | 27/10/2024 | FK Dukla Praha B | - | FK MAS Táborsko B | 2:3 | |
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| 13. Round | 01/11/2024 | FC Písek | - | FK Dukla Praha B | 1:1 | ||||
| 14. Round | 09/11/2024 | Dynamo České Budějovice B | - | FK Dukla Praha B | 2:1 |
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