| 16. Round | 08/03/2025 | FK Mladá Boleslav [Youth B] | - | 1. FC Slovácko [Youth B] | 1:4 | ||||
| 17. Round | 14/03/2025 | 1. FC Slovácko [Youth B] | - | FK Pardubice [Youth B] | 4:2 | ||||
| 20. Round | 05/04/2025 | Slovan Liberec [Youth B] | - | 1. FC Slovácko [Youth B] | 3:5 | ||||
| 21. Round | 12/04/2025 | 1. FC Slovácko [Youth B] | - | SFC Opava [Youth B] | 3:0 | ||||
| 22. Round | 18/04/2025 | AC Sparta Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 3:0 | ||||
| 24. Round | 03/05/2025 | 1. FC Slovácko [Youth B] | - | Sigma Olomouc [Youth B] | 2:3 | ||||
| 25. Round | 10/05/2025 | FC Zbrojovka Brno [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 | ||||
| 27. Round | 22/05/2025 | Dynamo České Budějovice [Youth B] | - | 1. FC Slovácko [Youth B] | 3:0 |
Czech Republic