| 1. Round | 10/08/2024 | Sokol Živanice | - | FK Kolín | 1:1 | ||||
| 3. Round | 25/08/2024 | Slovan Liberec B | - | FK Kolín | 3:2 | ||||
| 6. Round | 09/10/2024 | FK Kolín | - | Jiskra Ústí nad Orlicí | 0:0 | ||||
| 10. Round | 12/10/2024 | FK Kolín | - | SK Zápy | 0:1 | ||||
| 11. Round | 19/10/2024 | FK Pardubice B | - | FK Kolín | 7:0 | ||||
| 15. Round | 16/11/2024 | SK Kladno | - | FK Kolín | 0:2 | ||||
| 18. Round | 08/03/2025 | FK Kolín | - | Sokol Živanice | 3:0 dec. | ||||
| 25. Round | 27/04/2025 | FK Mlada Boleslav B | - | FK Kolín | 1:0 | ||||
| 26. Round | 03/05/2025 | FK Kolín | - | Arsenal Česká Lípa | 1:1 |  |
|||
| 33. Round | 07/05/2025 | Chlumec nad Cidlinou | - | FK Kolín | 1:3 | ||||
| 27. Round | 11/05/2025 | SK Zápy | - | FK Kolín | 2:2 | |
|||
| 28. Round | 17/05/2025 | FK Kolín | - | FK Pardubice B | 1:0 | ||||
| 34. Round | 21/05/2025 | FK Kolín | - | Sokol Brozany | 1:0 | ||||
| 29. Round | 24/05/2025 | FK Ústí nad Labem | - | FK Kolín | 3:1 | ||||
| 31. Round | 07/06/2025 | FK Baník Most-Souš | - | FK Kolín | 1:0 |
Czech Republic