| 16. Round | 08/03/2025 | Vysočina Jihlava [Youth B] | - | FC Zlín [Youth B] | 6:3 | ||||
| 17. Round | 14/03/2025 | Sigma Olomouc [Youth B] | - | Vysočina Jihlava [Youth B] | 5:2 | ||||
| 19. Round | 29/03/2025 | Banik Ostrava [Youth B] | - | Vysočina Jihlava [Youth B] | 9:0 | ||||
| 20. Round | 05/04/2025 | Vysočina Jihlava [Youth B] | - | Dynamo České Budějovice [Youth B] | 1:2 | ||||
| 21. Round | 12/04/2025 | FK Dukla Praha [Youth B] | - | Vysočina Jihlava [Youth B] | 4:1 | ||||
| 22. Round | 17/04/2025 | Vysočina Jihlava [Youth B] | - | Viktoria Plzeň [Youth B] | 0:1 | ||||
| 23. Round | 26/04/2025 | Vysočina Jihlava [Youth B] | - | AC Sparta Praha [Youth B] | 0:3 | ||||
| 24. Round | 03/05/2025 | FK Mladá Boleslav [Youth B] | - | Vysočina Jihlava [Youth B] | 3:2 | ||||
| 25. Round | 10/05/2025 | Vysočina Jihlava [Youth B] | - | FK Pardubice [Youth B] | 1:4 | ||||
| 27. Round | 23/05/2025 | Vysočina Jihlava [Youth B] | - | FK Jablonec [Youth B] | 3:0 | ||||
| 28. Round | 31/05/2025 | Slovan Liberec [Youth B] | - | Vysočina Jihlava [Youth B] | 5:1 | ||||
| 29. Round | 07/06/2025 | Vysočina Jihlava [Youth B] | - | SFC Opava [Youth B] | 4:2 |
Czech Republic