| 1. Round | 04/08/2023 | 1. FC Slovácko [Youth B] | - | FK Mladá Boleslav [Youth B] | 0:3 | ||||
| 3. Round | 19/08/2023 | 1. FC Slovácko [Youth B] | - | FC Zbrojovka Brno [Youth B] | 1:1 |  |
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| 4. Round | 29/08/2023 | Slavia Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 6:3 | ||||
| 5. Round | 02/09/2023 | 1. FC Slovácko [Youth B] | - | FK Frýdek-Místek [Youth B] | 2:0 | ||||
| 12. Round | 21/10/2023 | 1. FC Slovácko [Youth B] | - | FC Zlín [Youth B] | 0:3 | |
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| 11. Round | 25/10/2023 | FK Pardubice [Youth B] | - | 1. FC Slovácko [Youth B] | 5:2 | ||||
| 13. Round | 28/10/2023 | Dynamo České Budějovice [Youth B] | - | 1. FC Slovácko [Youth B] | 3:1 | ||||
| 6. Round | 14/11/2023 | Banik Ostrava [Youth B] | - | 1. FC Slovácko [Youth B] | 2:2 |  |
Czech Republic