| 1. Round | 03/08/2024 | ČSK Uherský Brod | - | FC Zbrojovka Brno B | 0:2 | ||||
| 2. Round | 11/08/2024 | FC Zbrojovka Brno B | - | FC Zlínsko | 4:0 | ||||
| 3. Round | 18/08/2024 | SK Uničov | - | FC Zbrojovka Brno B | 7:3 | ||||
| 4. Round | 25/08/2024 | FC Zbrojovka Brno B | - | FC Strání | 1:4 | ||||
| 6. Round | 08/09/2024 | FC Zbrojovka Brno B | - | TJ Unie Hlubina | 0:1 | ||||
| 10. Round | 06/10/2024 | FC Zbrojovka Brno B | - | FK Frýdek-Místek | 1:1 | ||||
| 11. Round | 12/10/2024 | FC Slovan Rosice | - | FC Zbrojovka Brno B | 1:2 |  |
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| 13. Round | 25/10/2024 | FC Hlučín | - | FC Zbrojovka Brno B | 3:1 | ||||
| 25. Round | 25/04/2025 | FK Hodonín | - | FC Zbrojovka Brno B | 4:3 | ||||
| 33. Round | 30/04/2025 | FC Zbrojovka Brno B | - | 1. SC Znojmo | 1:1 | ||||
| 26. Round | 04/05/2025 | FC Zbrojovka Brno B | - | 1. FC Slovácko B | 1:2 | ||||
| 27. Round | 10/05/2025 | FK Frýdek-Místek | - | FC Zbrojovka Brno B | 3:1 | |
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| 28. Round | 18/05/2025 | FC Zbrojovka Brno B | - | FC Slovan Rosice | 0:1 | ||||
| 29. Round | 24/05/2025 | TJ Start Brno | - | FC Zbrojovka Brno B | 1:1 |
Czech Republic