| 1. Round | 20/07/2024 | MFK Vyškov | - | 1. SK Prostějov | 0:0 |  |
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| 2. Round | 27/07/2024 | Sigma Olomouc B | - | 1. SK Prostějov | 0:0 | |
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| 3. Round | 31/07/2024 | 1. SK Prostějov | - | FC Zlín | 0:2 | ||||
| 4. Round | 04/08/2024 | Viktoria Žižkov | - | 1. SK Prostějov | 3:0 | ||||
| 5. Round | 09/08/2024 | 1. SK Prostějov | - | FC Sellier & Bellot Vlašim | 1:1 | ||||
| 8. Round | 01/09/2024 | Slavia Praha B | - | 1. SK Prostějov | 2:2 | ||||
| 11. Round | 28/09/2024 | 1. SK Prostějov | - | FK Varnsdorf | 1:1 | ||||
| 9. Round | 02/10/2024 | 1. SK Prostějov | - | Zbrojovka Brno | 3:1 | ||||
| 12. Round | 06/10/2024 | AC Sparta Praha B | - | 1. SK Prostějov | 5:2 | ||||
| 13. Round | 21/10/2024 | 1. SK Prostějov | - | MFK Chrudim | 1:1 | ||||
| 14. Round | 26/10/2024 | SK Líšeň | - | 1. SK Prostějov | 2:0 | ||||
| 15. Round | 01/11/2024 | 1. SK Prostějov | - | FK MAS Táborsko | 3:3 |
Czech Republic