| 2. Round | 12/08/2023 | Slovan Liberec [Youth B] | - | 1. FC Slovácko [Youth B] | 2:2 | ||||
| 3. Round | 19/08/2023 | 1. FC Slovácko [Youth B] | - | FC Zbrojovka Brno [Youth B] | 1:1 | ||||
| 4. Round | 29/08/2023 | Slavia Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 6:3 | ||||
| 5. Round | 02/09/2023 | 1. FC Slovácko [Youth B] | - | FK Frýdek-Místek [Youth B] | 2:0 | ||||
| 7. Round | 16/09/2023 | 1. FC Slovácko [Youth B] | - | MFK Karviná [Youth B] | 2:2 |  |
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| 8. Round | 23/09/2023 | Viktoria Plzeň [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 | ||||
| 16. Round | 02/03/2024 | FK Mladá Boleslav [Youth B] | - | 1. FC Slovácko [Youth B] | 3:3 |
Czech Republic