| 1. Round | 26/07/2009 | Dynamo České Budějovice | - | FK Baumit Jablonec | 1:1 |  |
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| 2. Round | 02/08/2009 | Bohemians Praha | - | Dynamo České Budějovice | 0:2 | ||||
| 3. Round | 09/08/2009 | FK Teplice | - | Dynamo České Budějovice | 3:0 | ||||
| 4. Round | 15/08/2009 | Dynamo České Budějovice | - | Slavia Praha | 0:1 | ||||
| 7. Round | 13/09/2009 | Bohemians 1905 | - | Dynamo České Budějovice | 2:0 | ||||
| 8. Round | 20/09/2009 | Dynamo České Budějovice | - | 1. FC Slovácko | 0:0 | ||||
| 11. Round | 17/10/2009 | AC Sparta Praha | - | Dynamo České Budějovice | 1:1 | ||||
| 12. Round | 25/10/2009 | Dynamo České Budějovice | - | Viktoria Plzeň | 0:0 | ||||
| 13. Round | 01/11/2009 | Sigma Olomouc | - | Dynamo České Budějovice | 2:0 | ||||
| 14. Round | 08/11/2009 | Dynamo České Budějovice | - | FK Mladá Boleslav | 1:3 |
Czech Republic