| 17. Round | 19/02/2001 | AC Sparta Praha | - | FC Stavo Artikel Brno | 1:0 |  |
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| 18. Round | 05/03/2001 | AC Sparta Praha | - | FK Jablonec 97 | 2:0 |  |
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| 19. Round | 09/03/2001 | Dynamo České Budějovice | - | AC Sparta Praha | 1:4 | |
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| 20. Round | 16/03/2001 | AC Sparta Praha | - | Slavia Praha | 4:4 | |
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| 21. Round | 02/04/2001 | FK Drnovice | - | AC Sparta Praha | 1:1 | |
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| 22. Round | 05/04/2001 | Viktoria Žižkov | - | AC Sparta Praha | 2:1 | ||||
| 23. Round | 09/04/2001 | AC Sparta Praha | - | 1. FC Synot Stare Mesto | 2:0 | ||||
| 24. Round | 16/04/2001 | Viktoria Plzeň | - | AC Sparta Praha | 2:3 | |
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| 26. Round | 29/04/2001 | Slovan Liberec | - | AC Sparta Praha | 1:1 | ||||
| 27. Round | 06/05/2001 | AC Sparta Praha | - | Baník Ostrava | 4:1 | |
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| 28. Round | 13/05/2001 | Chmel Blsany | - | AC Sparta Praha | 2:2 | ||||
| 29. Round | 18/05/2001 | FK Teplice | - | AC Sparta Praha | 2:3 | |
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| 30. Round | 25/05/2001 | AC Sparta Praha | - | Sigma Olomouc | 0:1 |
Czech Republic