| 18. Round | 09/03/2025 | TJ Start Brno | - | Hanácká Slávia Kroměříž | 0:1 | ||||
| 20. Round | 22/03/2025 | TJ Start Brno | - | FK Třinec | 2:1 | ||||
| 21. Round | 29/03/2025 | FK Hodonín | - | TJ Start Brno | 2:0 | ||||
| 22. Round | 05/04/2025 | TJ Start Brno | - | 1. FC Slovácko B | 0:1 | ||||
| 23. Round | 12/04/2025 | FK Frýdek-Místek | - | TJ Start Brno | 3:2 | ||||
| 24. Round | 17/04/2025 | TJ Start Brno | - | FC Slovan Rosice | 4:1 | ||||
| 25. Round | 25/04/2025 | TJ Start Brno | - | 1. SC Znojmo | 3:1 | ||||
| 33. Round | 30/04/2025 | TJ Start Brno | - | SK Uničov | 0:3 | ||||
| 26. Round | 03/05/2025 | FC Hlučín | - | TJ Start Brno | 1:3 | ||||
| 27. Round | 10/05/2025 | TJ Start Brno | - | MFK Karviná B | 2:4 | ||||
| 28. Round | 17/05/2025 | FC Zlín B | - | TJ Start Brno | 2:3 | ||||
| 29. Round | 24/05/2025 | TJ Start Brno | - | FC Zbrojovka Brno B | 1:1 |
Brazil