| 2. Round | 18/08/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 2:0 | ||||
| 17. Round | 28/08/2024 | FK Dukla Praha B | - | Viktoria Plzeň B | 2:0 |  |
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| 5. Round | 06/09/2024 | FC Chomutov | - | FK Dukla Praha B | 3:4 | |
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| 8. Round | 29/09/2024 | FK Dukla Praha B | - | Slovan Velvary | 1:1 | ||||
| 10. Round | 13/10/2024 | FK Dukla Praha B | - | Bohemians Praha 1905 B | 4:0 |  |
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| 13. Round | 01/11/2024 | FC Písek | - | FK Dukla Praha B | 1:1 |
Czech Republic