| 10. Round | 22/02/2025 | SFC Opava [Youth B] | - | Banik Ostrava [Youth B] | 1:1 |  |
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| 16. Round | 08/03/2025 | Dynamo České Budějovice [Youth B] | - | Banik Ostrava [Youth B] | 0:0 | ||||
| 17. Round | 14/03/2025 | Banik Ostrava [Youth B] | - | FK Dukla Praha [Youth B] | 4:1 | ||||
| 19. Round | 29/03/2025 | Banik Ostrava [Youth B] | - | Vysočina Jihlava [Youth B] | 9:0 | ||||
| 20. Round | 05/04/2025 | FK Mladá Boleslav [Youth B] | - | Banik Ostrava [Youth B] | 3:0 | ||||
| 22. Round | 09/04/2025 | Slavia Praha [Youth B] | - | Banik Ostrava [Youth B] | 2:1 | ||||
| 21. Round | 12/04/2025 | Banik Ostrava [Youth B] | - | FK Pardubice [Youth B] | 4:4 |  |
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| 23. Round | 25/04/2025 | Banik Ostrava [Youth B] | - | FK Jablonec [Youth B] | 7:2 | |
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| 24. Round | 03/05/2025 | Slovan Liberec [Youth B] | - | Banik Ostrava [Youth B] | 2:9 | ||||
| 18. Round | 07/05/2025 | Viktoria Plzeň [Youth B] | - | Banik Ostrava [Youth B] | 5:1 | ||||
| 25. Round | 10/05/2025 | Banik Ostrava [Youth B] | - | SFC Opava [Youth B] | 6:0 | |
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| 26. Round | 13/05/2025 | 1. FC Slovácko [Youth B] | - | Banik Ostrava [Youth B] | 2:2 |
Czech Republic