| 2. Round | 25/08/2024 | Dynamo České Budějovice [Women] | - | AC Sparta Praha B [Women] | 0:4 | ||||
| 3. Round | 31/08/2024 | AC Sparta Praha B [Women] | - | Baník Ostrava [Women] | 0:1 | ||||
| 4. Round | 07/09/2024 | Sigma Olomouc [Women] | - | AC Sparta Praha B [Women] | 3:4 | ||||
| 6. Round | 21/09/2024 | AC Sparta Praha B [Women] | - | FC Zlín [Women] | 4:0 | ||||
| 7. Round | 28/09/2024 | FK Dukla Praha [Women] | - | AC Sparta Praha B [Women] | 1:4 | ||||
| 8. Round | 05/10/2024 | AC Sparta Praha B [Women] | - | SK Líšeň [Women] | 7:2 | ||||
| 10. Round | 19/10/2024 | FK Teplice [Women] | - | AC Sparta Praha B [Women] | 1:7 |  |
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| 15. Round | 23/03/2025 | FC Zlín [Women] | - | AC Sparta Praha B [Women] | 1:3 | |
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| 16. Round | 30/03/2025 | AC Sparta Praha B [Women] | - | FK Dukla Praha [Women] | 7:0 | |
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| 17. Round | 12/04/2025 | SK Líšeň [Women] | - | AC Sparta Praha B [Women] | 0:3 | ||||
| 18. Round | 18/04/2025 | AC Sparta Praha B [Women] | - | Prague Raptors FC [Women] | 2:0 |
Slovakia