| 1. Round | 03/08/2024 | TJ Unie Hlubina | - | FC Slovan Rosice | 4:0 | ||||
| 2. Round | 11/08/2024 | TJ Start Brno | - | TJ Unie Hlubina | 1:1 | ||||
| 3. Round | 17/08/2024 | TJ Unie Hlubina | - | FC Hlučín | 1:4 | ||||
| 4. Round | 25/08/2024 | MFK Karviná B | - | TJ Unie Hlubina | 4:0 |  |
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| 5. Round | 31/08/2024 | TJ Unie Hlubina | - | FC Zlín B | 0:5 | ||||
| 6. Round | 08/09/2024 | FC Zbrojovka Brno B | - | TJ Unie Hlubina | 0:1 | ||||
| 9. Round | 28/09/2024 | TJ Unie Hlubina | - | FC Zlínsko | 2:1 | ||||
| 7. Round | 02/10/2024 | TJ Unie Hlubina | - | FK Blansko | 1:2 | ||||
| 10. Round | 06/10/2024 | SK Uničov | - | TJ Unie Hlubina | 4:1 | |
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| 11. Round | 12/10/2024 | TJ Unie Hlubina | - | FC Strání | 2:0 |  |
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| 12. Round | 19/10/2024 | Hanácká Slávia Kroměříž | - | TJ Unie Hlubina | 5:0 |
Czech Republic