| 18. Round | 09/03/2025 | FK Varnsdorf | - | Zbrojovka Brno | 0:3 |  |
|||
| 19. Round | 15/03/2025 | Zbrojovka Brno | - | FK MAS Táborsko | 0:2 | ||||
| 20. Round | 30/03/2025 | AC Sparta Praha B | - | Zbrojovka Brno | 1:1 | ||||
| 21. Round | 05/04/2025 | Zbrojovka Brno | - | MFK Vyškov | 1:2 | ||||
| 22. Round | 11/04/2025 | FC Zlín | - | Zbrojovka Brno | 1:1 | ||||
| 23. Round | 16/04/2025 | Zbrojovka Brno | - | 1. SK Prostějov | 1:1 | ||||
| 24. Round | 19/04/2025 | SK Líšeň | - | Zbrojovka Brno | 1:4 | ||||
| 25. Round | 26/04/2025 | Zbrojovka Brno | - | Sigma Olomouc B | 1:0 | ||||
| 26. Round | 02/05/2025 | FC Sellier & Bellot Vlašim | - | Zbrojovka Brno | 1:2 | |
|||
| 27. Round | 07/05/2025 | Zbrojovka Brno | - | Viktoria Žižkov | 4:1 | ||||
| 28. Round | 11/05/2025 | Slavia Praha B | - | Zbrojovka Brno | 1:1 | ||||
| 30. Round | 25/05/2025 | Zbrojovka Brno | - | Vysočina Jihlava | 1:1 |
Czech Republic