| 20. Round | 23/03/2025 | FK Dukla Praha B | - | FK Motorlet Praha | 5:0 | ||||
| 21. Round | 31/03/2025 | Bohemians Praha 1905 B | - | FK Dukla Praha B | 2:0 | ||||
| 23. Round | 13/04/2025 | FK MAS Táborsko B | - | FK Dukla Praha B | 0:3 | ||||
| 26. Round | 04/05/2025 | Viktoria Plzeň B | - | FK Dukla Praha B | 1:1 | ||||
| 32. Round | 07/05/2025 | FK Dukla Praha B | - | Dynamo České Budějovice B | 0:2 |  |
Czech Republic