| 1. Round | 04/08/2023 | 1. FC Slovácko [Youth] | - | FK Mladá Boleslav [Youth] | 1:5 | ||||
| 2. Round | 12/08/2023 | FK Mladá Boleslav [Youth] | - | Sigma Olomouc [Youth] | 1:1 | ||||
| 3. Round | 19/08/2023 | FK Pardubice [Youth] | - | FK Mladá Boleslav [Youth] | 3:3 |  |
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| 4. Round | 26/08/2023 | FK Mladá Boleslav [Youth] | - | FC Zlín [Youth] | 3:2 | ||||
| 5. Round | 02/09/2023 | Dynamo České Budějovice [Youth] | - | FK Mladá Boleslav [Youth] | 4:1 | ||||
| 6. Round | 09/09/2023 | FK Mladá Boleslav [Youth] | - | Vysočina Jihlava [Youth] | 1:3 | |
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| 9. Round | 30/09/2023 | FK Mladá Boleslav [Youth] | - | Slovan Liberec [Youth] | 4:0 | ||||
| 12. Round | 21/10/2023 | FK Frýdek-Místek [Youth] | - | FK Mladá Boleslav [Youth] | 1:1 |  |
Czech Republic