| 23. Round | 18/07/2021 | FC Machida Zelvia | - | SC Sagamihara | 1:0 | ||||
| 24. Round | 09/08/2021 | Kyoto Sanga FC | - | FC Machida Zelvia | 2:1 | ||||
| 25. Round | 14/08/2021 | FC Machida Zelvia | - | Fagiano Okayama | 1:1 | ||||
| 26. Round | 22/08/2021 | Giravanz Kitakyushu | - | FC Machida Zelvia | 2:1 | ||||
| 27. Round | 29/08/2021 | FC Machida Zelvia | - | Ventforet Kofu | 3:0 | ||||
| 28. Round | 04/09/2021 | Zweigen Kanazawa | - | FC Machida Zelvia | 0:4 | ||||
| 33. Round | 10/10/2021 | FC Machida Zelvia | - | JEF United Chiba | 0:1 | ||||
| 34. Round | 16/10/2021 | Blaublitz Akita | - | FC Machida Zelvia | 0:2 | ||||
| 35. Round | 24/10/2021 | FC Machida Zelvia | - | Tochigi SC | 0:0 |  |
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| 36. Round | 30/10/2021 | FC Ryūkyū | - | FC Machida Zelvia | 0:0 | ||||
| 37. Round | 03/11/2021 | FC Machida Zelvia | - | Matsumoto Yamaga | 3:2 | ||||
| 38. Round | 07/11/2021 | Ehime FC | - | FC Machida Zelvia | 0:0 | ||||
| 39. Round | 14/11/2021 | FC Machida Zelvia | - | Thespakusatsu Gunma | 1:1 | ||||
| 40. Round | 21/11/2021 | Montedio Yamagata | - | FC Machida Zelvia | 3:5 | ||||
| 41. Round | 28/11/2021 | FC Machida Zelvia | - | RB Omiya Ardija | 0:0 | ||||
| 42. Round | 05/12/2021 | Albirex Niigata | - | FC Machida Zelvia | 0:2 |
Japan