| 13. Round | 09/03/2025 | FK Dukla Praha [Women] | - | Dynamo České Budějovice [Women] | 2:0 | ||||
| 15. Round | 23/03/2025 | FK Dukla Praha [Women] | - | Sigma Olomouc [Women] | 2:3 | ||||
| 16. Round | 30/03/2025 | AC Sparta Praha B [Women] | - | FK Dukla Praha [Women] | 7:0 | ||||
| 17. Round | 13/04/2025 | FK Dukla Praha [Women] | - | FC Zlín [Women] | 2:3 | ||||
| 18. Round | 19/04/2025 | FC Hradec Králové [Women] | - | FK Dukla Praha [Women] | 2:0 |
Czech Republic