| 18. Round | 01/04/2025 | Slavia Praha [Youth] | - | 1. FC Slovácko [Youth] | 2:0 |  |
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| 21. Round | 12/04/2025 | FC Zbrojovka Brno [Youth] | - | Slavia Praha [Youth] | 0:3 | ||||
| 23. Round | 26/04/2025 | Dynamo České Budějovice [Youth] | - | Slavia Praha [Youth] | 1:1 | ||||
| 24. Round | 13/05/2025 | Slavia Praha [Youth] | - | FK Dukla Praha [Youth] | 3:5 |
Czech Republic