| 17. Round | 01/03/2025 | Zbrojovka Brno | - | MFK Chrudim | 0:0 | ||||
| 18. Round | 09/03/2025 | FK Varnsdorf | - | Zbrojovka Brno | 0:3 | ||||
| 19. Round | 15/03/2025 | Zbrojovka Brno | - | FK MAS Táborsko | 0:2 | ||||
| 21. Round | 05/04/2025 | Zbrojovka Brno | - | MFK Vyškov | 1:2 | ||||
| 22. Round | 11/04/2025 | FC Zlín | - | Zbrojovka Brno | 1:1 | ||||
| 23. Round | 16/04/2025 | Zbrojovka Brno | - | 1. SK Prostějov | 1:1 | ||||
| 24. Round | 19/04/2025 | SK Líšeň | - | Zbrojovka Brno | 1:4 |  |
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| 26. Round | 02/05/2025 | FC Sellier & Bellot Vlašim | - | Zbrojovka Brno | 1:2 | ||||
| 27. Round | 07/05/2025 | Zbrojovka Brno | - | Viktoria Žižkov | 4:1 | ||||
| 28. Round | 11/05/2025 | Slavia Praha B | - | Zbrojovka Brno | 1:1 | ||||
| 29. Round | 17/05/2025 | Zbrojovka Brno | - | Baník Ostrava B | 1:0 | |
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| 30. Round | 25/05/2025 | Zbrojovka Brno | - | Vysočina Jihlava | 1:1 |  |
Czech Republic