| 1. Round | 30/07/2000 | Viktoria Plzeň | - | Dynamo České Budějovice | 2:2 | ||||
| 2. Round | 06/08/2000 | Dynamo České Budějovice | - | CU Bohemians Praha | 0:1 | ||||
| 4. Round | 20/08/2000 | Dynamo České Budějovice | - | Baník Ostrava | 1:0 | ||||
| 5. Round | 27/08/2000 | AC Sparta Praha | - | Dynamo České Budějovice | 5:1 | ||||
| 10. Round | 13/10/2000 | Dynamo České Budějovice | - | Viktoria Žižkov | 2:2 | ||||
| 6. Round | 18/10/2000 | Dynamo České Budějovice | - | FK Teplice | 2:2 | ||||
| 11. Round | 22/10/2000 | FK Jablonec 97 | - | Dynamo České Budějovice | 1:1 | ||||
| 12. Round | 29/10/2000 | Chmel Blsany | - | Dynamo České Budějovice | 3:1 |  |
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| 8. Round | 01/11/2000 | Dynamo České Budějovice | - | FC Marila Příbram | 0:0 | ||||
| 13. Round | 04/11/2000 | Dynamo České Budějovice | - | Slavia Praha | 3:2 | |
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| 14. Round | 12/11/2000 | FK Drnovice | - | Dynamo České Budějovice | 2:0 | ||||
| 15. Round | 19/11/2000 | Dynamo České Budějovice | - | 1. FC Synot Stare Mesto | 1:1 | ||||
| 16. Round | 26/11/2000 | Dynamo České Budějovice | - | Viktoria Plzeň | 4:3 | |
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| 7. Round | 30/11/2000 | Sigma Olomouc | - | Dynamo České Budějovice | 2:1 | ||||
| 17. Round | 18/02/2001 | CU Bohemians Praha | - | Dynamo České Budějovice | 4:3 | |
Czech Republic