| 17. Round | 04/03/2023 | 1. SK Prostějov | - | FK Varnsdorf | 1:1 | ||||
| 18. Round | 13/03/2023 | Sigma Olomouc B | - | 1. SK Prostějov | 2:0 | ||||
| 19. Round | 18/03/2023 | 1. SK Prostějov | - | FK Viagem Příbram | 0:2 | ||||
| 20. Round | 01/04/2023 | MFK Chrudim | - | 1. SK Prostějov | 1:1 | ||||
| 21. Round | 08/04/2023 | 1. SK Prostějov | - | FC Sellier & Bellot Vlašim | 0:4 | ||||
| 22. Round | 16/04/2023 | FK MAS Táborsko | - | 1. SK Prostějov | 2:1 | ||||
| 23. Round | 19/04/2023 | 1. SK Prostějov | - | MFK Vyškov | 1:0 |  |
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| 24. Round | 23/04/2023 | AC Sparta Praha B | - | 1. SK Prostějov | 2:0 | ||||
| 25. Round | 29/04/2023 | 1. SK Prostějov | - | Vysočina Jihlava | 1:0 | ||||
| 26. Round | 07/05/2023 | MFK Karviná | - | 1. SK Prostějov | 5:0 | ||||
| 27. Round | 13/05/2023 | 1. SK Prostějov | - | SK Líšeň | 2:1 | ||||
| 28. Round | 19/05/2023 | SFC Opava | - | 1. SK Prostějov | 1:1 | ||||
| 29. Round | 24/05/2023 | FK Dukla Praha | - | 1. SK Prostějov | 4:1 | |
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| 30. Round | 28/05/2023 | 1. SK Prostějov | - | Slavia Praha B | 3:1 |
Slovakia