| 19. Round | 26/03/2025 | TJ Unie Hlubina | - | TJ Start Brno | 4:1 | ||||
| 21. Round | 29/03/2025 | FK Hodonín | - | TJ Start Brno | 2:0 | ||||
| 22. Round | 05/04/2025 | TJ Start Brno | - | 1. FC Slovácko B | 0:1 |  |
|||
| 25. Round | 25/04/2025 | TJ Start Brno | - | 1. SC Znojmo | 3:1 | ||||
| 26. Round | 03/05/2025 | FC Hlučín | - | TJ Start Brno | 1:3 | |
|||
| 27. Round | 10/05/2025 | TJ Start Brno | - | MFK Karviná B | 2:4 | |
|||
| 28. Round | 17/05/2025 | FC Zlín B | - | TJ Start Brno | 2:3 | ||||
| 29. Round | 24/05/2025 | TJ Start Brno | - | FC Zbrojovka Brno B | 1:1 | ||||
| 30. Round | 30/05/2025 | FK Blansko | - | TJ Start Brno | 3:0 |
Brazil