| 16. Round | 08/03/2025 | AC Sparta Praha [Youth B] | - | FK Jablonec [Youth B] | 7:0 | ||||
| 17. Round | 14/03/2025 | Dynamo České Budějovice [Youth B] | - | AC Sparta Praha [Youth B] | 5:2 | ||||
| 19. Round | 29/03/2025 | FK Dukla Praha [Youth B] | - | AC Sparta Praha [Youth B] | 2:4 | ||||
| 20. Round | 05/04/2025 | AC Sparta Praha [Youth B] | - | SFC Opava [Youth B] | 7:2 |  |
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| 21. Round | 12/04/2025 | Viktoria Plzeň [Youth B] | - | AC Sparta Praha [Youth B] | 1:2 | |
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| 22. Round | 18/04/2025 | AC Sparta Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 3:0 | ||||
| 23. Round | 26/04/2025 | Vysočina Jihlava [Youth B] | - | AC Sparta Praha [Youth B] | 0:3 | |
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| 24. Round | 03/05/2025 | AC Sparta Praha [Youth B] | - | FC Zlín [Youth B] | 3:1 | ||||
| 25. Round | 10/05/2025 | FK Mladá Boleslav [Youth B] | - | AC Sparta Praha [Youth B] | 2:4 | |
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| 26. Round | 17/05/2025 | AC Sparta Praha [Youth B] | - | Sigma Olomouc [Youth B] | 1:4 | |
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| 29. Round | 04/06/2025 | Slavia Praha [Youth B] | - | AC Sparta Praha [Youth B] | 2:2 |
Czech Republic