| 3. Round | 24/08/2024 | 1. FC Slovácko [Youth B] | - | Slavia Praha [Youth B] | 2:4 | ||||
| 4. Round | 29/08/2024 | FK Jablonec [Youth B] | - | 1. FC Slovácko [Youth B] | 2:6 | ||||
| 6. Round | 24/09/2024 | SFC Opava [Youth B] | - | 1. FC Slovácko [Youth B] | 3:1 | ||||
| 8. Round | 28/09/2024 | 1. FC Slovácko [Youth B] | - | FC Zlín [Youth B] | 0:1 | ||||
| 11. Round | 19/10/2024 | Banik Ostrava [Youth B] | - | 1. FC Slovácko [Youth B] | 7:5 | ||||
| 12. Round | 26/10/2024 | 1. FC Slovácko [Youth B] | - | Dynamo České Budějovice [Youth B] | 2:1 |
Czech Republic