| 1. Round | 04/08/2023 | 1. FC Slovácko [Youth B] | - | FK Mladá Boleslav [Youth B] | 0:3 |  |
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| 2. Round | 12/08/2023 | Slovan Liberec [Youth B] | - | 1. FC Slovácko [Youth B] | 2:2 | ||||
| 4. Round | 29/08/2023 | Slavia Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 6:3 | ||||
| 5. Round | 02/09/2023 | 1. FC Slovácko [Youth B] | - | FK Frýdek-Místek [Youth B] | 2:0 | ||||
| 8. Round | 23/09/2023 | Viktoria Plzeň [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 | ||||
| 9. Round | 30/09/2023 | 1. FC Slovácko [Youth B] | - | AC Sparta Praha [Youth B] | 1:7 | ||||
| 10. Round | 07/10/2023 | 1. FC Slovácko [Youth B] | - | Sigma Olomouc [Youth B] | 0:2 |
Ukraine