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| 1st(1st leg) | La Paz FC | - | San José | 0:1 (0:0) | ||
| 2nd (2nd leg) | San José | - | La Paz FC | 1:0 (0:0) | ||
| 1st(1st leg): 31/10/2009 19:30 | 2nd (2nd leg): 04/11/2009 00:00 | Winner: San José | ||||
| 1st(1st leg) | Club Aurora | - | Jorge Wilstermann | 1:3 (1:2) | ||
| 2nd (2nd leg) | Jorge Wilstermann | - | Club Aurora | 1:1 (0:0) | ||
| 1st(1st leg): 01/11/2009 19:00 | 2nd (2nd leg): 05/11/2009 00:00 | Winner: Jorge Wilstermann | ||||
| 1st(1st leg) | Real Mamoré | - | Universitario de Sucre | 0:0 (0:0) | ||
| 2nd (2nd leg) | Universitario de Sucre | - | Real Mamoré | 3:0 (3:0) | ||
| 1st(1st leg): 01/11/2009 20:00 | 2nd (2nd leg): 06/11/2009 00:00 | Winner: Universitario de Sucre | ||||
| 1st(1st leg) | The Strongest | - | Bolívar | 0:3 (0:0) | ||
| 2nd (2nd leg) | Bolívar | - | The Strongest | 2:3 (1:2) | ||
| 1st(1st leg): 01/11/2009 21:00 | 2nd (2nd leg): 05/11/2009 00:00 | Winner: Bolívar | ||||
| 1st(1st leg) | Oriente Petrolero | - | Blooming | 1:0 (0:0) | ||
| 2nd (2nd leg) | Blooming | - | Oriente Petrolero | 1:1 (1:1) | ||
| 1st(1st leg): 01/11/2009 23:00 | 2nd (2nd leg): 05/11/2009 00:30 | Winner: Oriente Petrolero | ||||
