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| 26/05/1959 | ADO | - | USV Elinkwijk | 2:1 (0:1) | ||
| 27/05/1959 | SC NEC | - | VV Zwartemeer | 1:2 (1:0, 1:1) aet | ||
| 28/05/1959 | NAC Breda | - | Fortuna '54 | 3:2 (0:0, 2:2, 2:2) pso | ||
| NOAD Tilburg | - | Rapid JC Heerlen | 2:5 (0:0) | |||
| SHS | - | AFC Ajax | 1:2 (0:0) | |||
| RC&FC Sparta | - | RKSV Volendam | 7:3 (3:3) | |||
| VVV | - | Enschedese Boys | 7:1 (3:0) | |||
| 30/05/1959 | vv Heerenveen | - | HVC Amersfoort | 1:4 (0:3) |
