| 4. Round | 31/08/2024 | Dunfermline Athletic | - | Ayr United | 1:1 | ||||
| 5. Round | 14/09/2024 | Ayr United | - | Partick Thistle | 1:1 | ||||
| 6. Round | 21/09/2024 | Queen's Park FC | - | Ayr United | 1:1 | ||||
| 7. Round | 28/09/2024 | Ayr United | - | Greenock Morton FC | 1:0 | ||||
| 8. Round | 05/10/2024 | Falkirk FC | - | Ayr United | 2:0 | ||||
| 9. Round | 19/10/2024 | Ayr United | - | Livingston FC | 1:2 | ||||
| 10. Round | 26/10/2024 | Airdrieonians FC | - | Ayr United | 0:1 | ||||
| 12. Round | 01/11/2024 | Raith Rovers | - | Ayr United | 2:0 | ||||
| 13. Round | 08/11/2024 | Greenock Morton FC | - | Ayr United | 1:1 | ||||
| 15. Round | 23/11/2024 | Ayr United | - | Dunfermline Athletic | 1:0 | ||||
| 16. Round | 07/12/2024 | Partick Thistle | - | Ayr United | 1:0 | ||||
| 17. Round | 14/12/2024 | Ayr United | - | Falkirk FC | 5:2 |  |
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| 18. Round | 21/12/2024 | Livingston FC | - | Ayr United | 0:1 |  |
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| 19. Round | 28/12/2024 | Ayr United | - | Greenock Morton FC | 0:0 |
Scotland