| 3. Round | 28/08/2016 | Sunderland AFC U23 | - | Southampton FC U23 | 3:0 | ||||
| 5. Round | 19/09/2016 | Southampton FC U23 | - | Leicester City U23 | 2:2 | ||||
| 6. Round | 23/09/2016 | Southampton FC U23 | - | Tottenham Hotspur U23 | 1:0 | ||||
| 7. Round | 14/10/2016 | Arsenal FC U23 | - | Southampton FC U23 | 0:0 | ||||
| 8. Round | 24/10/2016 | Southampton FC U23 | - | Manchester City U23 | 0:3 | ||||
| 9. Round | 31/10/2016 | Derby County U23 | - | Southampton FC U23 | 2:0 | ||||
| 10. Round | 21/11/2016 | Chelsea FC U23 | - | Southampton FC U23 | 3:2 |  |
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| 11. Round | 28/11/2016 | Southampton FC U23 | - | Everton FC U23 | 1:1 | ||||
| 14. Round | 06/01/2017 | Reading FC U23 | - | Southampton FC U23 | 4:2 | ||||
| 15. Round | 16/01/2017 | Southampton FC U23 | - | Arsenal FC U23 | 1:3 | ||||
| 16. Round | 30/01/2017 | Tottenham Hotspur U23 | - | Southampton FC U23 | 3:1 | ||||
| 18. Round | 20/02/2017 | Everton FC U23 | - | Southampton FC U23 | 2:0 | ||||
| 21. Round | 10/04/2017 | Southampton FC U23 | - | Liverpool FC U23 | 2:2 | ||||
| 22. Round | 21/04/2017 | Southampton FC U23 | - | Derby County U23 | 0:1 | ||||
| 13. Round | 05/05/2017 | Manchester City U23 | - | Southampton FC U23 | 4:2 |
England