| 28. Round | 13/03/2010 | Airdrie United FC | - | Ayr United | 1:1 |  |
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| 29. Round | 20/03/2010 | Ayr United | - | Queen Of The South | 3:0 | |
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| 34. Round | 17/04/2010 | Partick Thistle | - | Ayr United | 0:1 | |
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| 36. Round | 01/05/2010 | Greenock Morton FC | - | Ayr United | 2:1 | |
Scotland