| 17. Round | 28/02/2010 | FK Mladá Boleslav | - | SK Kladno | 0:1 |  |
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| 18. Round | 07/03/2010 | SK Kladno | - | AC Sparta Praha | 0:1 | ||||
| 19. Round | 14/03/2010 | FK Baumit Jablonec | - | SK Kladno | 1:0 | |
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| 20. Round | 20/03/2010 | SK Kladno | - | Viktoria Plzeň | 1:3 | |
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| 23. Round | 04/04/2010 | Bohemians Praha | - | SK Kladno | 1:4 |  |
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| 24. Round | 11/04/2010 | SK Kladno | - | Dynamo České Budějovice | 2:1 | |
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| 26. Round | 25/04/2010 | SK Kladno | - | 1. FC Slovácko | 1:0 | ||||
| 27. Round | 01/05/2010 | FK Teplice | - | SK Kladno | 5:0 | ||||
| 28. Round | 05/05/2010 | SK Kladno | - | Baník Ostrava | 0:3 | ||||
| 29. Round | 08/05/2010 | Bohemians 1905 | - | SK Kladno | 0:0 | |
Czech Republic