| 24. Round | 11/04/2010 | 1. FK Příbram | - | 1. FC Brno | 3:1 | ||||
| 26. Round | 24/04/2010 | FK Baumit Jablonec | - | 1. FC Brno | 1:0 | ||||
| 27. Round | 01/05/2010 | 1. FC Brno | - | Viktoria Plzeň | 2:2 | ||||
| 28. Round | 05/05/2010 | 1. FC Brno | - | Bohemians Praha | 0:0 | ||||
| 29. Round | 08/05/2010 | Dynamo České Budějovice | - | 1. FC Brno | 1:3 |  |
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| 30. Round | 15/05/2010 | 1. FC Brno | - | Bohemians 1905 | 1:2 |
Czech Republic