| 1. Round | 24/07/2021 | AC Sparta Praha | - | Sigma Olomouc | 3:2 | ||||
| 2. Round | 31/07/2021 | Sigma Olomouc | - | FK Pardubice | 3:2 |  |
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| 4. Round | 14/08/2021 | Sigma Olomouc | - | Dynamo České Budějovice | 3:3 |  |
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| 5. Round | 21/08/2021 | FC Fastav Zlín | - | Sigma Olomouc | 1:4 | |
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| 6. Round | 29/08/2021 | Sigma Olomouc | - | FK Jablonec | 4:0 | |
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| 7. Round | 12/09/2021 | FK Mladá Boleslav | - | Sigma Olomouc | 3:3 | |
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| 8. Round | 17/09/2021 | Sigma Olomouc | - | Baník Ostrava | 1:1 | |
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| 9. Round | 26/09/2021 | FK Teplice | - | Sigma Olomouc | 0:0 | ||||
| 10. Round | 02/10/2021 | Bohemians Praha 1905 | - | Sigma Olomouc | 2:0 | ||||
| 11. Round | 17/10/2021 | Sigma Olomouc | - | MFK Karviná | 2:0 | ||||
| 12. Round | 23/10/2021 | Slovan Liberec | - | Sigma Olomouc | 2:0 | ||||
| 3. Round | 27/10/2021 | Slavia Praha | - | Sigma Olomouc | 1:0 | |
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| 13. Round | 30/10/2021 | Sigma Olomouc | - | 1. FC Slovácko | 0:3 | ||||
| 14. Round | 06/11/2021 | Viktoria Plzeň | - | Sigma Olomouc | 0:0 |
Czech Republic