| 1. Round | 07/08/2009 | FC Eindhoven | - | MVV | 4:3 | ||||
| 2. Round | 13/08/2009 | MVV | - | HFC Haarlem | annull. | ||||
| 3. Round | 21/08/2009 | SC Cambuur | - | MVV | 1:1 |  |
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| 4. Round | 24/08/2009 | MVV | - | BV Veendam | 3:1 | ||||
| 5. Round | 28/08/2009 | RBC Roosendaal | - | MVV | 3:0 | ||||
| 6. Round | 04/09/2009 | MVV | - | FC Emmen | 2:1 | ||||
| 7. Round | 11/09/2009 | FC Den Bosch | - | MVV | 6:0 | ||||
| 8. Round | 18/09/2009 | MVV | - | SBV Excelsior | 1:1 | ||||
| 9. Round | 25/09/2009 | FC Oss | - | MVV | 3:2 | ||||
| 10. Round | 02/10/2009 | FC Zwolle | - | MVV | 2:1 | ||||
| 11. Round | 09/10/2009 | MVV | - | Telstar | 2:0 | ||||
| 12. Round | 16/10/2009 | MVV | - | Helmond Sport | 3:1 | ||||
| 13. Round | 23/10/2009 | De Graafschap | - | MVV | 2:1 | ||||
| 14. Round | 01/11/2009 | FC Dordrecht | - | MVV | 1:4 | ||||
| 15. Round | 06/11/2009 | MVV | - | FC Omniworld | 1:0 | ||||
| 16. Round | 13/11/2009 | MVV | - | FC Volendam | 5:2 | ||||
| 17. Round | 20/11/2009 | Go Ahead Eagles | - | MVV | 4:0 | ||||
| 18. Round | 27/11/2009 | MVV | - | AGOVV | 3:2 | ||||
| 19. Round | 30/11/2009 | Fortuna Sittard | - | MVV | 2:0 | ||||
| 20. Round | 04/12/2009 | Helmond Sport | - | MVV | 2:4 |
Belgium